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modified langendorff perfusion apparatus  (Kontes Glass)

 
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    Kontes Glass modified langendorff perfusion apparatus
    Modified Langendorff Perfusion Apparatus, supplied by Kontes Glass, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+langendorff+perfusion+apparatus/modified+langendorff+perfusion+apparatus/pm20449905-75-19-22
    Average 90 stars, based on 1 article reviews
    modified langendorff perfusion apparatus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Modification:

    Article Title: Free radicals mediate postshock contractile impairment in cardiomyocytes*
    Article Snippet: Electrical defibrillation is the only effective treatment for fatal ventricular arrhythmias.. However, the defibrillation itself results in myocardial injury, both morphologic and functional (1– 6).. Higher energy delivered during defibrillation results in more severe postresuscitation myocardial dysfunction (6–9).

    Article Title: High-energy defibrillation impairs myocyte contractility and intracellular calcium dynamics.
    Article Snippet: T he majority of patients initially resuscitated from cardiac arrest subsequently die before leaving the hospital (1, 2).. Postresuscitation myocardial dysfunction has been implicated as an important mechanism accounting for these fatal outcomes (3–9).. Studies in animals and in human patients have in fact extensively demonstrated impairment of ventricular function after resuscitation from cardiac arrest (1, 9–11).

    Article Title: Apoptosis, rather than oncosis, is the predominant mode of spontaneous death of isolated adult rat cardiac myocytes in culture.
    Article Snippet: Because of the high incidence of spontaneous cell death predominantly via apoptosis, this information is important for the interpretation of studies using this cell type in culture. (Jpn Circ J 2001; 65: 743–748) Key Words: Apoptosis; Cardiac myocytes; Necrosis; Oncosis; Ultrastructure T Japanese Circulation Journal Vol.65, August 2001 Sprague-Dawley rats (200–250 g) were injected with 500 units/kg heparin sodium intraperitoneally and then anesthetized with intraperitoneal pentobarbital (30 mg/kg). .. The heart was removed and hooked up to a modified Langendorff perfusion apparatus (Kontes) and washed for 5min with Ca2+-free modified Krebs-Ringer buffer containing (mmol/L) NaCl 110, KCl 2.6, MgSO4 1.2, KH2PO4 1.2, NaHCO3 25.0, HEPES 25.0, and glucose 11.0. .. The heart was then recirculated with buffer containing 0.1% collagenase type II (Worthington Biochemical Co) and 0.1% albumin.



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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
    Modified Langendorff Apparatus (Hse Ha Perfusion Systems, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    modified langendorff perfusion apparatus  (Kontes Glass)
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    modified langendorff perfusion apparatus kontes glass  (Kontes Glass)
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    Kontes Glass modified langendorff perfusion apparatus kontes glass
    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    modified langendorff heart perfusion apparatus  (Radnoti LLC)
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    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, <t>Langendorff-perfused</t> hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.
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    Image Search Results


    a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.

    Journal: Nature communications

    Article Title: The mitochondrial uniporter controls fight or flight heart rate increases

    doi: 10.1038/ncomms7081

    Figure Lengend Snippet: a–b, Immunofluorescent staining of ( a ) control right atrium and ( b ) gene painted SAN showing DAPI (blue) and HCN4 (red) to identify SAN, and Myc epitope tag (green) for DN-MCU-Myc expression. Scale bars are 200 μm. c–d, Example ex vivo ECG tracings recorded under basal (left) and ISO stimulated (right) conditions from gene painted hearts. Scale bar is 100 ms. e, Summary dose response data for ex vivo heart rate response to ISO. f , Percentage rate increases over baseline after ISO in gene-painted isolated, Langendorff-perfused hearts (data calculated from Fig. 2e). ***p<0.001, unpaired t test, n=22–24/group in panel e. Error bars indicate SEM.

    Article Snippet: Briefly, excised hearts from 7–9 week female and male mice were rapidly mounted on a modified Langendorff apparatus (HSE-HA perfusion systems, Harvard Apparatus, Holliston, Mass) for retrograde aortic perfusion at a constant pressure of 80 mm Hg with oxygenated (95% O 2 , 5% CO 2 ) Krebs-Henseleit solution consisting of (mM) 25.0 NaHCO 3 , 118.5 NaCl, 4.0 KCl, 1.2 MgSO 4 , 1.2 NaH 2 PO 4 , 1.5 CaCl 2 , and 11.2 glucose, with pH equilibrated to 7.4.

    Techniques: Staining, Expressing, Ex Vivo, Isolation